Silodosin is a selective α1a adrenergic antagonist approved for benign prostatic hypertrophy. Three simple, sensitive, precise and economical UV Spectrophotometric methods have been developed and validated in tablet formulation of Silodosin. Silodosin dissolved in 0.1 N HCl has shown absorption maxima at 273 nm (Method A). Method B is based on first order derivative spectra which showed a sharp peak at 265 nm. Calculation of Area under Curve (AUC) in Method C, gave absorption in the wavelength range of 268-278 nm. A good linear relationship (0.999) was observed between the concentration ranges of 2-120 μg/mL. The assay of silodosin tablet was found to be 99.22%. The molar absorptivity was found be 3.68 x 104 L mole-1 cm-1 in all three methods. The inter-day and intra-day precision was observed as 100.28 and 99.61 respectively. The LOD and LOQ values were obtained in the range 0.45-0.47 µg and 1.432-1.436 µg/ml respectively, that were within the limits. These methods are therefore found as simple, sensitivity, rapid, economical and suitable for the routine quality control application in pharmaceutical formulations.
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